Virtual Classroom, as a Support Tool for Face-To-Face Classes to Strengthen the Achievement of Significant Learning. A Support Strategy as a Means to Extend the Mathematics Classroom

Dada la importancia que están adquiriendo en la actualidad las metas establecidas en los Objetivos de Desarrollo Sostenible, las TIC constituyen un recurso invaluable para lograr el nivel educativo de nuestros niños, jóvenes y adultos en su conjunto. Este cuarto objetivo es la base para mejorar la calidad de vida, el acceso a una educación inclusiva y equitativa y el desarrollo sostenible de una educación de calidad.. El desarrollo de esta investigación respalda el trabajo incansable de los docentes para mantener a la vanguardia a nuestras instituciones educativas, quienes han logrado educar a un número importante de jóvenes en las nuevas tecnologías y aplicaciones educativas. Este estudio propone una adaptación a un entorno educativo virtual en la asignatura  Principios de Matematica  de la educación inicial-Matemática 105, crea un ecosistema digital con herramientas que pueden ser utilizadas en un entorno web, y apoyada por tutores capacitados y diversas teorías de aprendizaje adaptadas al entorno virtual.  Given the importance that the goals established in the Sustainable Development Goals are currently acquiring, ICTs constitute an invaluable resource to achieve the educational level of our children, young people and adults as a whole. This fourth objective is the basis for improving the quality of life, access to inclusive and equitable education and the sustainable development of quality education. The development of this research supports the tireless work of teachers to keep at the forefront our educational institutions, who have managed to educate a significant number of young people in new technologies and educational applications. This study proposes an adaptation to a virtual educational environment in the subject Principles of Mathematics of initial education-Mathematics 105, creates a digital ecosystem with tools that can be used in a web environment, and supported by trained tutors and various adapted learning theories to the virtual environment

Proposal for the Management of Digital Tools in University Contexts

Las herramientas digitales en los cursos de formación académica han cobrado importancia debido a la pandemia y los docentes no han estado preparados para desarrollar metodologías virtuales. Esto ha aumentado la urgencia de aprender a utilizar y compartir materiales audiovisuales para permitir enfoques sincrónicos y asincrónicos del proceso de aprendizaje. En cuanto a la investigación, fue proyectiva, utilizando un enfoque narrativo mixto. La población fue una muestra intencional de10 docentes y 87 estudiantes, con un total de 97 participantes. El estudio se realizó teniendo en cuenta las competencias técnicas propuestas por el Ministerio de Educación de Panamá (2014). Propone tres niveles: exploratorio, integrador e innovador, centrándose en las características, usos y oportunidades de las herramientas tecnológicas en el proceso educativo. A su vez, cada nivel cuenta con tres explicaciones que validan la metodología del docente. Teniendo en cuenta estos descriptores, fue necesario presentar una propuesta compuesta por tres módulos: Módulo 1, Motor de Recuperación de Información, para desarrollar la identificación de los diferentes motores de búsqueda. Módulo 2, Herramientas técnicas que habilitan la capacidad de utilizar herramientas digitales. El módulo 3 se ocupa del contenido del entorno virtual. El módulo tiene como función generar contenidos con los parámetros del marco legal para utilizar herramientas tecnológicas que mejoren las capacidades tecnológicas de los docentes con el objetivo de dinamizar las lecciones e involucrar a los estudiantes en el proceso educativoDigital tools in academic training courses have gained importance due to the pandemic and teachers have not been prepared to develop virtual methodologies. This has increased the urgency of learning to use and share audiovisual materials to enable synchronous and asynchronous approaches to the learning process. As for the research, it was projective, using a mixed narrative approach. The population was an intentional sample of 10 teachers and 87 students, with a total of 97 participants. The study was carried out taking into account the technical competencies proposed by the Ministry of Education of Colombia (2013). It proposes three levels: exploratory, integrative and innovative, focusing on the characteristics, uses and opportunities of technological tools in the educational process. In turn, each level has three explanations that validate the teacher's methodology. Taking these descriptors into account, it was necessary to present a proposal composed of three modules: Module 1, Information Retrieval Engine, to develop the identification of the different search engines. Module 2, Technical tools that enable the ability to use digital tools. Module 3 deals with the content of the virtual environment. The function of the module is to generate content with the parameters of the legal framework to use technological tools that improve the technological capabilities of teachers with the aim of energizing lessons and involving students in the educational process

Gestión del Departamento de Enfermería frente al abordaje del ausentismo, Centro Médico Paitilla, 2016-2019.

El siguiente proyecto se realizó en el Centro Médico Paitilla. con el objetivo de fortalecer la gestión del Departamento de Enfermería de dicha institución, para el abordaje del ausentismo laboral. mediante la aplicación de un plan operativo. La población de estudio se concretó con el personal de Enfermería del Centro Médico Paitilla: Enfermeras gerentes y rotadoras, y Técnicos en Enfermería con un total de 213. Cabe señalar que este tema del proyecto fue sugerido por el Departamento de Enfermería del Centro Médico Paitilla. tomando en cuenta la necesidad de sensibilizar al personal de Enfermería de la importancia en la asistencia para lograr el balance entre las actividades laborales y la vida familiar. Como resultado de la aplicación de las estrategias para disminuir el ausentismo Laboral, se pudo conocer que el mismo disminuyó positivamente en un 20.5% para las Enfermeras y un 16,6% en los Técnicos en Enfermería

Scoliosis induced by medullary damage: an experimental study in rabbits

To date, there have been no reports of experiments designed to induce scoliosis by direct damage of different areas of the spinal cord. In a series of rabbits with medullary damage, the authors attempted selectively to interrupt the pathways that mediate proprioceptive input. Unilateral lesion of the dorsal column and posterior horn of the spinal cord was performed using three different techniques: coagulation with laser, stereotaxic microcoagulation, and longitudinal electrocoagulation. Of 32 operated rabbits, 17 developed scoliosis, exhibiting clear pathologic damage of the spinal cord. Electrophysiologic study, including EMG and analysis of the tonic -- vibratory reflex, was performed on 10 rabbits with medullary damage (scoliotic and non-scoliotic) and 12 matched controls. The data suggest disturbance of the sensory afferences that control the postural tone and consequent muscular imbalance, expressed as reduced activity in the muscles of the convex side. This work supports the view that loss of proprioceptive neural impulses caused by medullary damage can induce scoliosi

CRISPR-ERA for Switching Off (Onco) Genes

Genome-editing nucleases like the popular CRISPR/Cas9 enable the generation of knockout cell lines and null zygotes by inducing site-specific double-stranded breaks (DSBs) within a genome. In most cases, when a DNA template is not present, the DSB is repaired by nonhomologous end joining (NHEJ), resulting in small nucleotide insertions or deletions that can be used to construct knockout alleles. However, for several reasons, these mutations do not produce the desired null result in all cases, instead generating a similar protein with functional activity. This undesirable effect could limit the therapeutic efficiency of gene therapy strategies focused on abrogating oncogene expression by CRISPR/Cas9 and should be taken into account. This chapter reviews the irruption of CRISPR technology for gene silencing and its application in gene therapy

Endoperiodontal lesions: diagnosis first, then treatment and not always tooth extraction: a cross-sectional survey in Spain and a proposal of a clinical treatment protocol

Background: Endoperiodontal lesion (EPL) is defined as a pathological communication between pulpal and periodontal tissues. Currently, accurate diagnosis and treatment of this pathology are challenging. This study aims to identify the different endoperiodontal therapies to propose a clinical protocol to simplify and unify the criteria for EPL treatment. Material and Methods: Observational cross-sectional study through an electronic survey. This study matches STROBE guidelines. The anonymous questionnaire contained open-ended and close-ended questions and was distributed to dentistry professors of the UPV/EHU and different professionals from Spanish associations and scientific societies. The data collected were analyzed using descriptive and analytical statistics. Results: A total of 128 responses were obtained, of which 120 were active professionals or had not been so for less than 5 years. The majority of professionals were women (65.6%) and from the Basque Country (63.9%). A total of 86.6% reported having complementary studies to a degree or a bachelor’s degree. The treatments performed by these professionals were similar to those reported in the literature, which started with root canal treatment when there was an endodontic origin (91.5%), and with basic periodontal treatment when periodontal (51.3%). Conclusions: Considering the current scientific evidence and the clinical practice of professionals in the treatment of EPL, we designed a clinical protocol. This protocol needs validation in larger populations and with longer follow-ups

Targeting the JAK/STAT Pathway: A Combined Ligand- And Target-Based Approach

Janus kinases (JAKs) are a family of proinflammatory enzymes able to mediate the immune responses and the inflammatory cascade by modulating multiple cytokine expressions as well as various growth factors. In the present study, the inhibition of the JAK-signal transducer and activator of transcription (STAT) signaling pathway is explored as a potential strategy for treating autoimmune and inflammatory disorders. A computationally driven approach aimed at identifying novel JAK inhibitors based on molecular topology, docking, and molecular dynamics simulations was carried out. For the best candidates selected, the inhibitory activity against JAK2 was evaluated in vitro. Two hit compounds with a novel chemical scaffold, 4 (IC50 = 0.81 μM) and 7 (IC50 = 0.64 μM), showed promising results when compared with the reference drug Tofacitinib (IC50 = 0.031 μM).This study was funded by the University of Valencia and Generalitat Valenciana (GVA) through postdoctoral grants no. UVINV_POSTDOC18-785681 and APOSTD/2019/055 (M.G-L.) and by the University of Bologna through research grant no. RFO2019 (P.R., S.C., and M.R.)

Mechanism of sulfur transfer across protein-protein interfaces: The cysteine desulfurase model system

CsdA cysteine desulfurase (the sulfur donor) and the CsdE sulfur acceptor are involved in biological sulfur trafficking and in iron-sulfur cluster assembly in the model bacterium Escherichia coli. CsdA and CsdE form a stable complex through a polar interface that includes CsdA Cys328 and CsdE Cys61, the two residues known to be involved in the sulfur transfer reaction. Although mechanisms for the transfer of a sulfur moiety across protein-protein interfaces have been proposed based on the IscS-IscU and IscS-TusA structures, the flexibility of the catalytic cysteine loops involved has precluded a high resolution view of the active-site geometry and chemical environment for sulfur transfer. Here, we have used a combination of X-ray crystallography, solution NMR and SAXS, isothermal calorimetry, and computational chemistry methods to unravel how CsdA provides a specific recognition platform for CsdE and how their complex organizes a composite functional reaction environment. The X-ray structures of persulfurated (CsdA) and persulfurated (CsdA-CsdE) complexes reveal the crucial roles of the conserved active-site cysteine loop and additional catalytic residues in supporting the transpersulfuration reaction. A mechanistic view of sulfur transfer across protein-protein interfaces that underpins the requirement for the conserved cysteine loop to provide electrostatic stabilization for the in-transfer sulfur atom emerges from the analysis of the persulfurated (CsdA-CsdE) complex structure.BFU2008-02372/BMC, CSD 2006-23, and BFU2011-22588 to M.C., CTQ2012-36253-C03-03 and CTQ2015-66223-C2 to I.T., CTQ2015-64597-C2-1-P to J.J.B., and BFU2010-22266- C02-02 and CTQ2015-66206-C2-2-R to M.C.V. Further support for this work was obtained from the Generalitat Valenciana (ACOMP/2015/239 to I.T.) and from the European Commission FP7 ComplexINC grant (contract no. 279039) to M.C.V.Peer Reviewe

The mechanism of the transpersulfuration reaction in a cysteine desulfurase-sulfur acceptor model system

Trabajo presentado en las 1as Jornadas Españolas de Biocatálisis, celebradas en Madrid (España) del 02 al 03 de julio de 2015.Escherichia coli CsdA cysteine desulfurase (the sulfur donor) and the CsdE sulfur acceptor are involved in biological sulfur trafficking, in iron-sulfur cluster assembly, and tRNA hypermodification [1] in the model bacterium Escherichia coli. CsdA and CsdE form a stable complex through a polar interface. Although mechanisms for the transfer of a sulfur moiety across protein-protein interfaces have been proposed based on the IscS-IscU and IscS-TusA structures [2,3], the flexibility of the catalytic Cys loops involved has precluded a high resolution view of the active-site geometry and chemical environment responsible to facilitate sulfur transfer. Here, we have used a combination of X-ray crystallography, solution NMR, biophysical and computational chemistry methods to unravel how CsdA provides a specific recognition platform for CsdE and how their complex organizes a composite functional reaction environment. A mechanistic view of sulfur transfer across protein-protein interfaces emerges from the structural analysis of the CSD system

Cardiotrophin-1 promotes a high survival rate in rabbits with lethal fulminant hepatitis of viral origin

Rabbit hemorrhagic disease virus (RHDV) causes lethal fulminant hepatitis closely resembling acute liver failure (ALF) in humans. In this study, we investigated whether cardiotrophin-1 (CT-1), a cytokine with hepatoprotective properties, could attenuate liver damage and prolong survival in virus-induced ALF. Twenty-four rabbits were infected with 2 × 10(4) hemagglutination units of RHDV. Twelve received five doses of CT-1 (100 μg/kg) starting at 12 h postinfection (hpi) (the first three doses every 6 h and then two additional doses at 48 and 72 hpi), while the rest received saline. The animals were analyzed for survival, serum biochemistry, and viral load. Another cohort (n = 22) was infected and treated similarly, but animals were sacrificed at 30 and 36 hpi to analyze liver histology, viral load, and the expression of factors implicated in liver damage and repair. All infected rabbits that received saline died by 60 hpi, while 67% of the CT-1-treated animals survived until the end of the study. Treated animals showed improved liver function and histology, while the viral loads were similar. In the livers of CT-1-treated rabbits we observed reduction of oxidative stress, diminished PARP1/2 and JNK activation, and decreased inflammatory reaction, as reflected by reduced expression of tumor necrosis factor alpha, interleukin-1β, Toll-like receptor 4, VCAM-1, and MMP-9. In addition, CT-1-treated rabbits exhibited marked upregulation of TIMP-1 and increased expression of cytoprotective and proregenerative growth factors, including platelet-derived growth factor B, epidermal growth factor, platelet-derived growth factor receptor β, and c-Met. In conclusion, in a lethal form of acute viral hepatitis, CT-1 increases animal survival by attenuating inflammation and activating cytoprotective mechanisms, thus representing a promising therapy for ALF of viral origin